IGF1 then activates the p38-dependent JAK–STAT1 axis to promote M(IFN-γ) polarization and suppress STAT6-mediated M(IL-4) activation. Mechanistically, serine metabolism deficiency increases the expression of IGF1 by reducing the promoter abundance of S-adenosyl methionine-dependent histone H3 lysine 27 trimethylation. Here, we show that suppressing serine metabolism, either by inhibiting the activity of the key enzyme phosphoglycerate dehydrogenase in the serine biosynthesis pathway or by exogenous serine and glycine restriction, robustly enhances the polarization of interferon-γ-activated macrophages (M(IFN-γ)) but suppresses that of interleukin-4-activated macrophages (M(IL-4)) both in vitro and in vivo. Not only does it allow you to run detailed and thorough analyses, but it also provides you with the tools to check the resulting data for errors by retracing each performed step.Serine metabolism is reportedly involved in immune cell functions, but whether and how serine metabolism regulates macrophage polarization remain largely unknown. Therefore, regardless of whether you are working with quantitative or qualitative data, the app includes several templates where you can get started and have peace of mind that you are entering the data correctly, choosing the appropriate analyses and graphs.Īll in all, GraphPad Prism is a great tool for anyone who deals with managing scientific data. Allows you to organize your data efficientlyĪ noteworthy feature of the program is that it is specifically formatted for the analyses you want to run.
In all fairness, the tool is not intended solely for statisticians, although having basic knowledge about the significance of the performed calculations is somewhat mandatory. The utility includes numerous types of analysis, from common to very specific, including here linear and nonlinear regression, ANOVA, binary logistic regression or T Tests, just to name a few. At the same time, the spreadsheet-like interface allows you to visualize the data in a structured manner, while providing quick access to all the tools you need. The application bundles a wide array of features and tools designed to guide you through all the necessary steps of organizing the information and performing detailed statistical analysis. Therefore, you can get started by opening your own file or you can simply start from scratch by choosing one of the projects. The application comes with a user-friendly interface and fine-tuned documentation where you can learn tips and tricks suitable for both beginners and advanced users. Comes with an extensive library of analyses GraphPad Prism is a robust application packed with a full-featured, comprehensive biostatistics features that aims to assist biologists and researchers in analyzing complex sets of data and generating understandable graphs.
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